Device

Part:BBa_K672001:Design

Designed by: Emmanuel Garuz   Group: iGEM11_UTP-Panama   (2011-09-28)

New Promoter For Periplasmic Heat Generation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 322
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As the part K328000 is a basic part, instead of a combination of subparts (Promoter, etc...), we had to analyse the sequence, cut out the HybB promoter and combine manually with the CsPA sequence.


Source

Part:BBa_K410000 (hybB-ompA-AOX1A)
Part:BBa_K328001 (CsPA promoter)

References

CsPA Promoter

  • Fang L., Jiang W., Bae W., Inouye M., 1997, Promoter-independent cold-shock induction of cspA and its derepression at 37 degrees C by mRNA stabilization., Mol Microbiol. 23(2):355-64
  • Hankins JS., Zappavigna C., Prud'homme-Genereux A., Mackie GA., 2007, Role of RNA structure and susceptibility to RNase E in regulation of a cold shock mRNA, cspA mRNA., J Bacteriol. 189(12):4353-8
  • Xia B., Ke H., Jiang W., Inouye M., 2002, The Cold Box stem-loop proximal to the 5'-end of the Escherichia coli cspA gene stabilizes its mRNA at low temperature., J Biol Chem. 277(8):6005-11

OmpA + Aox